John Engelhardt, Ph.D. (Full Member)
Professor, University of Iowa, Department of Internal Medicine
Director, Center for Gene Therapy of Cystic Fibrosis and Other Genetic Diseases
Department Chair, University of Iowa Department of Anatomy and Cell Biology
Professor, University of Iowa, Department of Anatomy and Cell Biology

http://elab.genetics.uiowa.edu/

Research Interest    

Research in the Engelhardt laboratory focuses on the molecular basis of inherited and environmentally induced diseases, and on the development of gene therapies for these disorders. Included are two major research areas: 1) the study of lung molecular and cellular biology as it relates to the pathogenesis and treatment of cystic fibrosis (CF) lung disease, and 2) the molecular mechanism underlying redox-mediated injury and the development of molecular therapies for ischemia/reperfusion injury, sepsis, and the neurodegenerative disease amyotrophic lateral sclerosis (ALS). Research on lung biology includes the study of adult epithelial stem cells and their niches in the airway, and dissecting the pathogenesis of lung disease in animal models of cystic fibrosis. The laboratory is currently elucidating transcriptional signals important for early establishment of the airway glandular stem cell niche, and for mobilization of stem cells from this niche following airway injury. The newly generated ferret and pig models of cystic fibrosis are being used to understand how stem cells in the adult airway respond to injury resulting from dysfunction of CFTR (the chloride channel that is defective in cystic fibrosis). These new genetic models of CF are also being used to dissect pathophysiologic mechanisms of disease and to develop gene therapies that target the lung with recombinant adeno-associated virus. A second area of interest is the development of pharmacologic and gene-based therapies for redox-dependent disorders of the liver and spinal cord. Such studies are aimed at the treatment of environmentally induced liver injuriesthose resulting from sepsis, ischemia/reperfusion, and (in the case of ALS) motor neuron injury. We are using rodent transgenic and knockout models to better understand the mechanisms that are involved in inflammation and injury and are controlled by reactive oxygen species. Of particular interest in this area are mechanisms of redox-regulated NF?B activation via several NADPH oxidase complexes (Nox1, Nox2 and Nox4), whose roles include generating intracellular superoxides in response to extracellular pro-inflammatory cytokine signals (IL-1beta and TNFalpha pathways are a major focus). This redox-signaling program heavily utilizes recombinant viral vectors, transgenic/knockout mice, and proteomic approaches to address basic aspects of pathophysiology, and also to generate new therapies capable of promoting organ regeneration and repair while also minimizing the deleterious inflammatory responses to injury.

Selected publications:

Liu, X, Luo, M, Guo, C, Yan, Z, Wang, Y, Lei-Butters, DC, and Engelhardt, JF. Analysis of adeno-associated virus progenitor cell transduction in mouse lung. Mol Ther 17:285-293, 2009.

Sun X, Yan Z, Yi Y, Li Z, Lei D, Rogers CS, Chen J, Zhang Y, Welsh MJ, Leno GH, and Engelhardt JF. Adeno-associated virus-targeted disruption of the CFTR gene in cloned ferrets. J Clin Invest 118: 1578-1583, 2008.

Harraz MM, Marden JJ, Zhou W, Zhang Y, Williams A, Sharov VS, Nelson K, Luo M, Paulson H, Schoneich C, and Engelhardt JF. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J Clin Invest 118: 659-670, 2008.

Marden JJ, Zhang Y, Oakley FD, Zhou W, Luo M, Jia HP, McCray PB, Jr, Yaniv M, Weitzman JB, and Engelhardt JF. JunD protects the liver from ischemia/reperfusion injury by dampening AP-1 transcriptional activation. J Biol Chem 283: 6687-6695, 2008.

Mumbengegwi DR, Li Q, Li C, Bear CE, and Engelhardt JF. Evidence for a superoxide permeability pathway in endosomal membranes. Mol Cell Biol 28: 3700-3712, 2008.

Publications from Gene Therapy Center

Publications from PubMed